Coding

Part:BBa_K1911003:Design

Designed by: Jack Kwon   Group: iGEM16_LambertGA   (2016-10-09)


ClpX from E. Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 879
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 361
    Illegal AgeI site found at 1099
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There were illegal restriction sites which cut the ClpX gene into smaller sequences, rendering it obsolete. So we had to introduce wobbles to eliminate these illegal restriction sites so that ClpX may be expressed and allowed to function as a complete protein.


Source

ClpX is found naturally in the E. coli genome.

References